Application
Western blot
Sample
Caenorhabditis elegans Cell lysate - whole cell (whole worm lysate)
Gel Running Conditions
Reduced Denaturing (SDS-Page 10%)
Loading amount
80 µg
Specification
whole worm lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST containing 5% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/1500
Name
Non-Abcam antibody was used: anti rabbit from Santa Cruz
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Positive control
Mouse liver sample
Additional data
Additional Notes
This test is to use a promotion code to detect FOXO1 protein in C.elegans sample. There is no band on indicated size as company claims but a strong band about 64kD for both c.elegans and mouse liver. We never tried mouse liver sample before and I don't know whether it is detectable with this antibody. If other researcher already did mouse liver and it is around 64 KD then I believe the band I observed from C.elegans is FOXO1 protein. Otherwise, FOXO1 is not detectable with this primary antibody
Abcam response
Thank you so much for testing this antibody in C. elegans.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work in this species. The customer received a discount on a future antibody purchase for testing this product in C. elegans. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Lorenzo Ramos-Mucci
Verified customer
提交于 Dec 12 2012