Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
(agent) for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1% Block Ace
Secondary antibody
Name
Non-Abcam antibody was used: FITC-AffiniPure Donkey Anti-Goat IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Abcam response
Thank you for your valuable feedback. We are sorry to hear that this antibody has not given you good results in Immunocytochemistry (ICC/IF). This antibody has not been tested in this application before, and it is possible that it is not suitable for use. However, we would be unable to confirm this without further testing of the antibody in the application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
啓憲 杉峰
Verified customer
提交于 Apr 18 2011