Application
Western blot
Sample
Mouse Cell lysate - whole cell (ES cell)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE gel)
Loading amount
20 µg
Specification
ES cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% blocking buffer
Dilution
1/5000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 100 kDa
Positive control
human H1299 whole cell lysate 20ug
(this sample was loaded at different membrane)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 02 2013