Application
Western blot
Sample
Human Cell lysate - whole cell (human fibroblast and stem cell)
Loading amount
50 µg
Specification
human fibroblast and stem cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Secondary antibody
Name
Non-Abcam antibody was used: HRP goat anti-rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 50 kDa
Additional data
Additional Notes
lane 1 human fibroblast
lane 2 human ES cell
upper band represented FOXG1
bottom band reprented actin
lane 2 human ES cell
upper band represented FOXG1
bottom band reprented actin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 10 2012