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Our Abpromise guarantee covers the use of ab23738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.7 - 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 49 kDa).|
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ChIP/Chip||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration.|
|EMSA||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
(Image courtesy of Human Protein Atlas) ab23738 staining FOXA1 in Human prostate. Paraffin embedded human prostate tissue was incubated with ab23738 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23738 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ICC/IF image of ab23738 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23738 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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