概述

  • 产品名称
  • 描述
    兔多克隆抗体to FLIP
  • 特异性
    Recognizes all FLIP splice variants including FLIP alpha, beta, gamma, and delta.
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human FLIP aa 2-19.
    Sequence:

    SAEVIHQVEEALDTDEKE


    (Peptide available as ab8457)

性能

应用

Our Abpromise guarantee covers the use of ab8421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 55 kDa.Can be blocked with Human FLIP peptide (ab8457).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
  • 组织特异性
    Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
  • 序列相似性
    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • 结构域
    The caspase domain lacks the active sites residues involved in catalysis.
  • 翻译后修饰
    Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c FLIP antibody
    • c FLIPL antibody
    • c FLIPR antibody
    • c FLIPS antibody
    • c-FLIP antibody
    • c-FLIPL antibody
    • c-FLIPR antibody
    • c-FLIPS antibody
    • CASH antibody
    • CASP8 and FADD like apoptosis regulator antibody
    • CASP8 and FADD-like apoptosis regulator subunit p12 antibody
    • CASP8AP1 antibody
    • Caspase homolog antibody
    • Caspase like apoptosis regulatory protein antibody
    • Caspase related inducer of apoptosis antibody
    • Caspase-eight-related protein antibody
    • Caspase-like apoptosis regulatory protein antibody
    • Casper antibody
    • Cellular FLICE-like inhibitory protein antibody
    • CFLA antibody
    • Cflar antibody
    • CFLAR_HUMAN antibody
    • CLARP antibody
    • FADD like anti apoptotic molecule antibody
    • FADD-like antiapoptotic molecule 1 antibody
    • FLAME antibody
    • FLAME-1 antibody
    • FLAME1 antibody
    • FLIP antibody
    • I FLICE antibody
    • I-FLICE antibody
    • Inhibitor of FLICE antibody
    • MACH-related inducer of toxicity antibody
    • MRIT antibody
    • OTTHUMP00000163715 antibody
    • OTTHUMP00000206475 antibody
    • OTTHUMP00000206476 antibody
    • OTTHUMP00000206478 antibody
    • OTTHUMP00000206479 antibody
    • OTTHUMP00000206480 antibody
    • OTTHUMP00000206482 antibody
    • OTTHUMP00000207360 antibody
    • Usurpin antibody
    • Usurpin beta antibody
    see all

Anti-FLIP antibody 图像

  • Lane 1 : Anti-FLIP antibody (ab8421) at 1 µg/ml
    Lane 2 : Anti-FLIP antibody (ab8421) at 2 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate


    Predicted band size : 55 kDa
  • Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 µg/ml.

  • ab8421 at 5µg/ml staining FLIP in Hela cells by ICC/IF 

Anti-FLIP antibody (ab8421)参考文献

This product has been referenced in:
  • Jeudy S  et al. Bcl-2 Inhibits the Innate Immune Response during Early Pathogenesis of Murine Congenital Muscular Dystrophy. PLoS One 6:e22369 (2011). WB ; Mouse . Read more (PubMed: 21850221) »
  • Järvinen K  et al. Caspase-8, c-FLIP, and caspase-9 in c-Myc-induced apoptosis of fibroblasts. Exp Cell Res 317:2602-15 (2011). Read more (PubMed: 21903094) »

See all 4 Publications for this product

Product Wall

Thank you for getting back to me.

The peptide would be free, and it would be sent through Tecnolab. As it will be delivered by them I am unsure of how long it will take. The peptide is in stock and would be sent out as soon as possible. I wil...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))
Loading amount
0.5 µg
Specification
Rabbit Reticulocyte
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 02 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount
500000 cells
Specification
Canine Kidney Epithelial MDCK cells
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 11 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (A549 (human lung epithelium cells))
Total protein in input
1e+007 cells
Immuno-precipitation step
Other - Protein G Dynabeads
Specification
A549 (human lung epithelium cells)
Username

Abcam user community

Verified customer

提交于 Nov 05 2016

Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (VERO E6)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount
75000 cells
Specification
VERO E6
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 22 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (A549 (human lung epithelium cells))
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount
75000 cells
Specification
A549 (human lung epithelium cells)
Blocking step
No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 13 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts (MEFs))
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount
25000 cells
Specification
Mouse embryonic fibroblasts (MEFs)
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 23 2016

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver, hepatocytes)
Specification
liver, hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 05 2015

That is no problem. I'm glad you have found it useful.

Once you have received the peptide, please do let me know how you get on. Until then, I wish you all the best with your research.

Sorry for the delay in getting back to you.

I have some further information to share with you in regards to anti-FLIP antibody (ab8421). I have attached the quality control western blots obtained using this antibody. As can be seen from the w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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