Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (Exponential growth)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
10 µg
Specification
Exponential growth
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBST
Dilution
1/10000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey-anti-Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
FKBP12 deletion strain
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 12 kDa Non-specific: 11, 53 kDa
Positive control
FKBP12-TAP tagged strain
Additional data
Additional Notes
Unspecific band detected at MW ~ 52kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 09 2015