Application
Western blot
Sample
Dog Cell lysate - whole cell (Human cervical cancer (HeLa), Canine osteosarcoma)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Human cervical cancer (HeLa), Canine osteosarcoma
Blocking step
T20 Super Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/4000
Secondary antibody
Dilution
1/40000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 second(s)
Bands
Specific: 145 kDa
Positive control
HeLa cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 16 2018