Application
Immunohistochemistry (Frozen sections)
Blocking step
1% BSA and 5%NGS as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Tissue sections (Embryonic brain tissue)
Specification
Embryonic brain tissue
Permeabilization
Yes - Heated Citrate Buffer pH 6.0
Fixative
Formaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%BSA + 1%NGS + 0.3% Saponin in PBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen Alexa Fluor 488 goat anti-rabbit IgG (H
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
Tried anti-FGF8 antibody with and without heat mediated antigen retrieval (95C citrate buffer pH 6.0 for 15 min) also tried it with and without 2nd postfix in 4%PFA. There is an unexpected signal surrounding oral cavity (not seen in no-primary-control) but no specific staining within the brain.
Abcam response
Thank you so much for testing this antibody in mouse and IHC-Fr.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species and application.
We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Michael Rudy
Verified customer
提交于 May 16 2014