FCCP,mitochondrial oxidative phosphorylation uncoupler (ab120081)
Key features and details
- Potent mitochondrial oxidative phosphorylation uncoupler
- CAS Number: 370-86-5
- Purity: > 99%
Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
FCCP,mitochondrial oxidative phosphorylation uncoupler -
描述
Potent mitochondrial oxidative phosphorylation uncoupler -
生物学描述
Potent mitochondrial oxidative phosphorylation uncoupler (IC50 = 20 nM). Disrupts ATP synthesis by transporting protons across mitochondrial inner membranes. Depolarises mitochondrial membrane potential.
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纯度
> 99% -
CAS编号
370-86-5 -
化学结构
性能
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化学名称
Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone
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分子量
254.17 -
PubChem识别号
3330 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 100 mM
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处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
FC(F)(F)Oc1ccc(cc1)NN=C(/C#N)C#N -
来源
Synthetic
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研究领域
相关产品
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Alternative Versions
图片
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2D chemical structure image of ab120081, FCCP, mitochondrial oxidative phosphorylation uncoupler
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HT29 cells were treated with the indicated compounds ((etoposide, 10 µM; gemcitabine 0.1 µM; paclitaxel 0.01 µM; PD901 1 µM, VX-680 0.2 µM) for 24 hours before analysis of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) using the Seahorse XF96 extracellular flux analyzer. Baseline rates (black) were determined at the indicated times before the addition of oligomycin (green) and then FCCP (red). Rate data are normalized to per-well cell number determined by post-analysis high-content imaging.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (38)
ab120081 被引用在 38 文献中.
- Da Silva A et al. N-acetylneuraminate pyruvate lyase controls sialylation of muscle glycoproteins essential for muscle regeneration and function. Sci Adv 9:eade6308 (2023). PubMed: 37390204
- Sautchuk R et al. Transcriptional regulation of cyclophilin D by BMP/Smad signaling and its role in osteogenic differentiation. Elife 11:N/A (2022). PubMed: 35635445
- Moya ELJ et al. Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells. Int J Pharm 621:121780 (2022). PubMed: 35504427
- Hekman KE et al. Autophagy Enhances Longevity of Induced Pluripotent Stem Cell-Derived Endothelium via mTOR-Independent ULK1 Kinase. Stem Cells Transl Med 11:1151-1164 (2022). PubMed: 36173887
- Moya ELJ et al. Miniaturization and Automation of a Human In Vitro Blood-Brain Barrier Model for the High-Throughput Screening of Compounds in the Early Stage of Drug Discovery. Pharmaceutics 13:N/A (2021). PubMed: 34208550