概述

  • 产品名称
    Anti-FANCL抗体
    参阅全部 FANCL 一抗
  • 描述
    兔多克隆抗体to FANCL
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Dog
  • 免疫原

    Synthetic peptide from a region within internal sequence amino acids 144-193 (SGREHLITLK LKAKYPAESP DYFVDFPVPF CASWTPQVNS PQSSLISIYS) of Human FANCL (NP_001108108).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab94458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
  • 通路
    Protein modification; protein ubiquitination.
  • 疾病相关
    Fanconi anemia complementation group L
  • 序列相似性
    Contains 1 RING-type zinc finger.
  • 结构域
    The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.
  • 翻译后修饰
    The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • E3 ubiquitin-protein ligase FANCL antibody
      • FAAP43 antibody
      • Fancl antibody
      • FANCL_HUMAN antibody
      • Fanconi anemia complementation group L antibody
      • Fanconi anemia group L protein antibody
      • Fanconi anemia-associated polypeptide of 43 kDa antibody
      • FLJ10335 antibody
      • PHD finger protein 9 antibody
      • PHF9 antibody
      • POG antibody
      • Ubiquitin ligase protein FANCL antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCL antibody (ab94458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCL antibody (ab94458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling FANCL with ab94458 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm and nuclei of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - FANCL. Right - Merge.
    • Western blot - Anti-FANCL antibody (ab94458)
      Western blot - Anti-FANCL antibody (ab94458)
      Anti-FANCL antibody (ab94458) at 1 µg/ml + Human fetal heart lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 43 kDa



      Gel concentration: 12%
    • Immunocytochemistry/ Immunofluorescence - Anti-FANCL antibody (ab94458)
      Immunocytochemistry/ Immunofluorescence - Anti-FANCL antibody (ab94458)This image is courtesy of an anonymous Abreview
      ab94458 staining FANCL in untreated HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 (0.5%) and blocked with 5% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/500 in PBS + 5% BSA + 0.05% Tween20) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    文献

    This product has been referenced in:
    • Jamsai D  et al. Loss of GGN leads to pre-implantation embryonic lethality and compromised male meiotic DNA double strand break repair in the mouse. PLoS One 8:e56955 (2013). WB ; Mouse . Read more (PubMed: 23451117) »
    • Jamsai D  et al. Uncoupling of transcription and translation of Fanconi anemia (FANC) complex proteins during spermatogenesis. Spermatogenesis 5:e979061 (0). Read more (PubMed: 26413409) »

    See all 2 Publications for this product

    发表研究结果有使用 ab94458？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    2 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-FANCL antibody

     Below Average
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HepDES 19 cell)
    Loading amount
    10000 cells
    Specification
    HepDES 19 cell
    Treatment
    pcDNA3.1 or pcDNA-FANCL
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    abreview image
    Username

    Dr. Shijian Zhang

    Verified customer

    提交于 Nov 07 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FANCL antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.5% Triton X-100
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Oct 28 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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