Anti-FANCI抗体(ab74332)
Key features and details
- Rabbit polyclonal to FANCI
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FANCI抗体
参阅全部 FANCI 一抗 -
描述
兔多克隆抗体to FANCI -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat -
免疫原
Synthetic peptide corresponding to a region between residue 1025 and 1075 of human KIAA1794 (NP_060663.2)
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阳性对照
- Whole cell lysates from HeLa and 293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74332于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 149 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 149 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites. -
疾病相关
Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. -
结构域
The C-terminal 30 residues are probably required for function in DNA repair. -
翻译后修饰
Monoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
Phosphorylated in response to DNA damage by ATM and/or ATR. -
细胞定位
Nucleus. Concentrates in nuclear foci upon genotoxic stress. - Information by UniProt
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数据库链接
- Entrez Gene: 55215 Human
- Omim: 611360 Human
- SwissProt: Q9NVI1 Human
- Unigene: 513126 Human
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别名
- FANCI antibody
- FANCI gene antibody
- FANCI_HUMAN antibody
see all
图片
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All lanes : Anti-FANCI antibody (ab74332) at 0.04 µg/ml
Lane 1 : Whole HeLa cell lysate at 50 µg
Lane 2 : Whole HeLa cell lysate at 15 µg
Lane 3 : Whole HeLa cell lysate at 5 µg
Lane 4 : Whole 293T cell lysate at 50 µg
Predicted band size: 149 kDa
Additional bands at: 100 kDa, 65 kDa. We are unsure as to the identity of these extra bands. -
Detection of Human KIAA1794 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab74332 at 3 µg/mg for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents IgG control IP.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab74332 被引用在 5 文献中.
- Zhang J et al. UBE2T regulates FANCI monoubiquitination to promote NSCLC progression by activating EMT. Oncol Rep 48:N/A (2022). PubMed: 35703356
- Jaber S et al. p53 downregulates the Fanconi anaemia DNA repair pathway. Nat Commun 7:11091 (2016). WB . PubMed: 27033104
- Luebben SW et al. A concomitant loss of dormant origins and FANCC exacerbates genome instability by impairing DNA replication fork progression. Nucleic Acids Res 42:5605-15 (2014). ICC/IF ; Mouse . PubMed: 24589582
- Jamsai D et al. Loss of GGN leads to pre-implantation embryonic lethality and compromised male meiotic DNA double strand break repair in the mouse. PLoS One 8:e56955 (2013). WB ; Mouse . PubMed: 23451117
- Luebben SW et al. Helq acts in parallel to Fancc to suppress replication-associated genome instability. Nucleic Acids Res N/A:N/A (2013). PubMed: 24005041