Anti-FAM49B抗体(ab121299)
Key features and details
- Rabbit polyclonal to FAM49B
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FAM49B抗体 -
描述
兔多克隆抗体to FAM49B -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to amino acids 2-134 of Human FAM49B (Q9NUQ9).
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阳性对照
- RT4, U-251 MG and Human Tonsil lysate; Human kidney tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121299于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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说明 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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序列相似性
Belongs to the FAM49 family. - Information by UniProt
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数据库链接
- Entrez Gene: 51571 Human
- SwissProt: Q9NUQ9 Human
- Unigene: 126941 Human
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别名
- BM 009 antibody
- FA49B_HUMAN antibody
- Fam49b antibody
see all
图片
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All lanes : Anti-FAM49B antibody (ab121299) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : Human Plasma lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus. Recommended concentration of ab121299 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121299 at 1/100 dilution staining FAM49B in Paraffin-emebedded Human kidney tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab121299 被引用在 1 文献中.
- Zhang Y et al. TASP1 Promotes Gallbladder Cancer Cell Proliferation and Metastasis by Up-regulating FAM49B via PI3K/AKT Pathway. Int J Biol Sci 16:739-751 (2020). PubMed: 32071545