Anti-FAK (phospho Y925)抗体(ab38512)
Key features and details
- Rabbit polyclonal to FAK (phospho Y925)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FAK (phospho Y925)抗体
参阅全部 FAK 一抗 -
描述
兔多克隆抗体to FAK (phospho Y925) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human FAK aa 850-950 (phospho Y925).
Database link: Q05397 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab38512于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 123 kDa (predicted molecular weight: 119 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 123 kDa (predicted molecular weight: 119 kDa). |
靶标
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功能
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. -
组织特异性
Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
结构域
The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. -
翻译后修饰
Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. -
细胞定位
Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions. - Information by UniProt
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数据库链接
- Entrez Gene: 5747 Human
- Omim: 600758 Human
- SwissProt: Q05397 Human
- Unigene: 395482 Human
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别名
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
see all
图片
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All lanes : Anti-FAK (phospho Y925) antibody (ab38512) at 1/500 dilution
Lane 1 : HepG2 cell lysate, pre-incubated with phosphopeptide.
Lane 2 : HepG2 cell lysate.
Lane 3 : 293 cell lysate + EGF + serum.
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L).
Predicted band size: 119 kDa
Observed band size: 123 kDa why is the actual band size different from the predicted?
Lanes can be loaded with 5-30µg of total protein.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab38512 被引用在 8 文献中.
- Higuchi M et al. Paradoxical activation of c-Src as a drug-resistant mechanism. Cell Rep 34:108876 (2021). PubMed: 33761359
- Chai Z et al. Recombinant Viral Capsid Protein L2 (rVL2) of HPV 16 Suppresses Cell Proliferation and Glucose Metabolism via ITGB7/C/EBPß Signaling Pathway in Cervical Cancer Cell Lines. Onco Targets Ther 12:10415-10425 (2019). PubMed: 31819523
- Cui S et al. Genistein inhibits the growth and regulates the migration and invasion abilities of melanoma cells via the FAK/paxillin and MAPK pathways. Oncotarget 8:21674-21691 (2017). PubMed: 28423510
- Gill MB et al. KSHV-TK is a tyrosine kinase that disrupts focal adhesions and induces Rho-mediated cell contraction. EMBO J 34:448-65 (2015). PubMed: 25471072
- Bernusso VA et al. Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells. Biochim Biophys Acta 1853:388-95 (2015). PubMed: 25450971
- Tomioka H et al. Novel anti-microbial peptide SR-0379 accelerates wound healing via the PI3 kinase/Akt/mTOR pathway. PLoS One 9:e92597 (2014). WB ; Rat . PubMed: 24675668
- Wu M et al. Mesenchymal stem cells with modification of junctional adhesion molecule a induce hair formation. Stem Cells Transl Med 3:481-8 (2014). PubMed: 24558164
- Gaspar M et al. Murid herpesvirus-4 exploits dendritic cells to infect B cells. PLoS Pathog 7:e1002346 (2011). ICC/IF ; Mouse . PubMed: 22102809