Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
HeLa Cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in PBS
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: DAKO Polyclonal Swine anti Rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: Approximately 139 kDa Non-specific: Approximately 135 kDa
Additional data
Additional Notes
6X 5 min wash with TBS-T after primary and secondary antibody incubation. Two bands present in all experiments.
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提交于 May 13 2016