Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH8.6, 100C, 20min
Sample
Human Tissue sections (placenta)
Specification
placenta
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent, background reducers
Dilution
1/300
Secondary antibody
Name
Non-Abcam antibody was used: Leica Refine anti-mouse, anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
automated on Leica Bond III various pre-treatment regims tested. EDTA gave best signal to background
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提交于 Sep 12 2014