Application
Western blot
Sample
Mouse Cell lysate - whole cell (A549 (mouse liver cell line), H4IIE (rat hepatoma)
Loading amount
30 µg
Specification
A549 (mouse liver cell line), H4IIE (rat hepatoma
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Other product details
Dilution
1/100
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: HRP-conjugated anti-mouse secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 64 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Dongil Kim
Verified customer
提交于 May 03 2013