Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Parasaggital section of brain)
Specification
Parasaggital section of brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/100
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: Dako anti mouise Igs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Submitted images give same results as Elphick's 1998 paper?
Additionally, I note that what appear to be oligodendrocytes are positive ( lower image).This Ab can be further diluted.
Additionally, I note that what appear to be oligodendrocytes are positive ( lower image).This Ab can be further diluted.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Jan 08 2009