Ethanol Assay试剂盒(ab65343)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 10 nM
概述
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产品名称
Ethanol Assay试剂盒
参阅全部 Ethanol 试剂盒 -
检测方法
Colorimetric/Fluorometric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Quantitative -
灵敏度
> 10 nM -
范围
2 µM - 200 µM -
检测时间
1h 00m -
产品概述
Ethanol Assay Kit ab65343 provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media.
In the ethanol assay protocl, alcohol oxidase oxidizes ethanol to generate H2O2 which reacts with a probe to generate color (absorbance max 570 nm) and fluorescence (Ex/Em 535/587 nm).
The kit detects 0.1-10 ppm alcohol (~10-800 nM).
Ethanol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at 37ºC
- analyze with microplate reader -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K620 Ethanol Colorimetric/Fluorometric Assay Kit. K620-100 is the same size as the 100 test size of ab65343.
PLEASE NOTE: Extreme care should be taken to ensure that no alcohol vapors (ethanol, methanol, propanol) are in the laboratory air where this assay is to be performed. Alcohol vapors in the air will be rapidly absorbed by kit components resulting in very high background making the kit unusable. Laboratories where HPLC equipment and solvents are standing or where alcohol is used to wipe down laboratory benches or equipment are inappropriate locations to perform this assay.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 2000 tests Wash Buffer I 1 x 25ml 20 x 25ml Ethanol Enzyme Mix 1 vial 20 vials Ethanol Standard 1 x 500µl 20 x 500µl OxiRed Probe 1 x 200µl 20 x 200µl -
研究领域
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相关性
Alcohol (ethanol C2H5OH) is a clear colorless liquid rapidly absorbed from the gastrointestinal tract and distributed through the body. Ethanol has a depressive effect on the central nervous system and because of its psychoactive effects, it is considered a drug. Ethanol is metabolized by the body as an enerfy-providing carbohydrate nutrient, as it metabolized into acetyl CoA, an intermediate common with glucose than can be used for energy in the citric acid cycle (TCA) or for biosynthesis. Ethanol within the human body is converted into acetaldehyde by alcohol dehydrogenase and then into acetic acid by acetaldehyde dehydriogenase. Acetaldehyde, the product of the first step of the alcohol breakdown, is linked to most of the toxic clinical effects of alcohol. -
别名
- Ethyl alcohol
图片
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Ethanol in blood plasma was analyzed using ethanol assay kit (ab65343). Ethanol concentrations were determined 60 minutes after urethane administration in P0/1 mice and rats.
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0.5 nmol of ethanol was added to diluted human biological fluids (1/10) and spiked samples were measured fluorimetrically (background subtracted, duplicates; +/- SD).
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5 nmol of ethanol was added to diluted human biological fluids (1/10) and spiked samples were measured colourimetrically (background subtracted, duplicates; +/- SD).
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Standard curves with background signal subtracted (duplicates; +/- SD).
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Ethanol Standard Curve (Background subtracted). Expected background (0 nmole EtOH standard) for the standard curve can be around ~0.6-0.7 OD, with a typical dynamic range of 0.7-1.2 after background subtraction. Ethanol standard was diluted as described and the assay was performed according to the kit instructions.
数据表及文件
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SDS download
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Datasheet download
文献 (37)
ab65343 被引用在 37 文献中.
- Hsueh CY et al. A positive feed-forward loop between Fusobacteriumnucleatum and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis. iScience 25:103829 (2022). PubMed: 35198889
- Hulsebus HJ et al. Ethanol Intoxication Impairs Respiratory Function and Bacterial Clearance and Is Associated With Neutrophil Accumulation in the Lung After Streptococcus pneumoniae Infection. Front Immunol 13:884719 (2022). PubMed: 35603143
- Kong Q et al. Reducing alcohol and/or cocaine-induced reward and toxicity via an epidermal stem cell-based gene delivery platform. Mol Psychiatry 26:5266-5276 (2021). PubMed: 33619338
- Farooq A et al. The Role of Phosphate in Alcohol-Induced Experimental Pancreatitis. Gastroenterology 161:982-995.e2 (2021). PubMed: 34051238
- Tsuchiya Y et al. Physiological Role of Aerobic Fermentation Constitutively Expressed in an Aluminum-Tolerant Cell Line of Tobacco (Nicotiana tabacum). Plant Cell Physiol 62:1460-1477 (2021). PubMed: 34184745