Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic stem cell)
Specification
embryonic stem cell
Fixative
methanol:acetone 1:1
Permeabilization
No
Blocking step
0.1%BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/100
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG 488 alexa fluor
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
Staining was achieved using methanol/acetone fixation however, significantly different staining was seen using 4% paraformaldehyde fixation. The staining seen using methanol/acetone is similar to previously published immunofluorescence PMID18179891 and is therefore assumed to be correct.
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提交于 Nov 24 2011