Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7, Monocytes and Monocyte-Derived-Macrophages)
Loading amount
25 µg
Specification
MCF7, Monocytes and Monocyte-Derived-Macrophages
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 30 minute(s) · Temperature: 24°C · Diluent: 5% Milk, 1xTBS, 0.1% tween
Secondary antibody
Name
Non-Abcam antibody was used: Goat-anti-mouse (H+L) HRP-Conjugated
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
chemiluminesence
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 66 and 46 kDa
Additional data
Additional Notes
This antibody also recognizes the truncated 46kDa of the human ERa in addition to the 66 kDa full-length ERa in MCF7 breast cancer cells, as well as in human peripheral blood monocytes and monocyte-derived-macrophages
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提交于 Apr 16 2008