Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (vagina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
vagina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: PBS plus 1xcasein
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: biotin_goat@rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
this antibody did not label the nerve bundle in vagina tissue, while ab66102 showed strong cross-reaction.
Abcam response
Horse is an untested species for this antibody, considering the data customer have submitted, it seems like the antibody is not specific with Horse samples. However we will also look forward to hear from other customers before making any decision about horse cross reactivity. Please contact Scientific Support department using for any inquiries.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 26 2015