重组
RabMAb

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187)抗体[EP197Y] - BSA and Azide free (ab214171)

概述

  • 产品名称
    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187)抗体[EP197Y] - BSA and Azide free
    参阅全部 Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一抗
  • 描述
    兔单克隆抗体[EP197Y] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) - BSA and Azide free
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). Swissprot Numbers: P27361, P28482.

  • 阳性对照
    • WB: A431 (treated with EGF), SH-SY5Y (treated with NGF), PC-12 (treated with NGF), HeLa and NIH/3T3 whole cell lysates. IP: A431 (treated with EGF) and PC-12 (treated with NGF) whole cell lysates.
  • 常规说明

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab214171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 43, 42 kDa (predicted molecular weight: 43, 41 kDa).
IP Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 文献

    This product has been referenced in:
    • Zhang J  et al. Comparisons of ethanol extracts of chinese propolis (poplar type) and poplar gums based on the antioxidant activities and molecular mechanism. Evid Based Complement Alternat Med 2015:307594 (2015). Mouse . Read more (PubMed: 25802536) »
    • Xu W  et al. Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis. Biol Reprod 89:142 (2013). WB ; Mouse . Read more (PubMed: 24198121) »

    See all 7 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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