Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast cancer cell lines)
Permeabilization
Yes - PBS 0.5% Triton-X 5 minutes
Specification
Breast cancer cell lines
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/150
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Thermofisher Anti-Mouse IgG (H+L) Cross-Adsorbed S
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
Breast cancer cell lines with published HER4 mRNA and protein expression were used as control and antibody validation. In IF, ab19391 produced cytoplasmic and nuclear staining. There was little difference in fluorescence intensities among the breast cancer cell lines attempted.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 05 2017