Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Rat Cell lysate - whole cell (cultured Schwann cells)
Specification
cultured Schwann cells
Treatment
10 ng/ml GCF for 30 mins (lane 2)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2% skim milk in PBS
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: HRP conjugated anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
West Dura
Bands
Specific: 200 kDa Non-specific: 55, 60, 90, 120 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
Lane 1 : Schwann cell extract untreated
Positive control
Lane 2: Schwann cell extract treated with GGF
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提交于 Sep 04 2013