Application
Western blot
Sample
Human Cell lysate - whole cell (N87 cells)
Gel Running Conditions
Reduced Denaturing (10% Tris Glycin gel)
Loading amount
400000 cells
Specification
N87 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% milk in TBST
Concentration
0.5 µg/ml
Detection
Detection method
ECL
Exposure
1 second(s)
Bands
Specific: 138 kDa
Positive control
Those cells are known to be positive for ErbB 2
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提交于 Jun 22 2016