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Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. H3 (methyl K27) is also thought to have an important role in the repression of HOX genes during development and in X chromosome inactivation and imprinting. H3 (mono-methyl K27),a modification enriched at pericentromeric heterochromatin, was observed to be broadly distributed throughout all euchromatic sites and participates in silencing gene expression.
EpiSeeker Histone H3 (mono-methyl K27) Quantification Kit (Colorimetric) (ab115068) allows the user to specifically measure global mono-methylation of histone H3-K27 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|8-Well Sample Strips (with Frame)||4 units||9 units|
|8-Well Standard Control Strips||2 units||3 units|
|ESC1 (10X Wash Buffer)||1 x 10ml||1 x 20ml|
|ESC2 (Antibody Buffer)||1 x 6ml||1 x 12ml|
|ESC3 (Detection Antibody, 1 mg/ml)||1 x 5µl||1 x 10µl|
|ESC4 (Color Developer)||1 x 5ml||1 x 10ml|
|ESC5 (Stop Solution)||1 x 3ml||1 x 6ml|
|Standard Control (100 µg/ml)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115068 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"