EpiSeeker Histone H3 (mono-methyl K27) Quantification Kit (Colorimetric) (ab115068)

Overview

  • Product nameEpiSeeker Histone H3 (mono-methyl K27) Quantification Kit (Colorimetric)参阅全部 Histone H3 (K27 methylation) 试剂盒 ...
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Sensitivity
    2 ng/well
  • Range
    20 ng/well - 5000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. H3 (methyl K27) is also thought to have an important role in the repression of HOX genes during development and in X chromosome inactivation and imprinting. H3 (mono-methyl K27),a modification enriched at pericentromeric heterochromatin, was observed to be broadly distributed throughout all euchromatic sites and participates in silencing gene expression.

    EpiSeeker Histone H3 (mono-methyl K27) Quantification Kit (Colorimetric) (ab115068) allows the user to specifically measure global mono-methylation of histone H3-K27 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115068 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    EpiSeeker Histone H3 (mono-methyl K27) Quantification Kit (Colorimetric) images

    • ab115068 used to measure the amount of mono-methyl H3-K27. Histone extracts were prepared from MDA-MB-231 cells using the EpiSeeker Histone Extraction Kit (ab113476).

    Protocols

    References for EpiSeeker Histone H3 (mono-methyl K27) Quantification Kit (Colorimetric) (ab115068)

    ab115068 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"