Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Treatment
stimulated with 1´g/ml clustered ephrinb1-Fc
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-tween-5% BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: GAR_HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 110 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
lane 1 : Hela cells
lane 3 : HeLa cells overexpressing EphB2-kinase dead (K661R)
Positive control
lane 2 : HeLa cells overexpressing EphB2
Additional data
Additional Notes
All cells were stimulated with preclustered ephrinb1-Fc chimeric protein.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Thomas Jungas
Verified customer
提交于 Apr 11 2014