Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
No
Sample
Mouse Cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Gating Strategy
live cells in fsc vs ssc
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.2% BSA, 0.1%NaAz in PBS
Sample buffer: 0.2% BSA, 0.1%NaAz in PBS
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: 0.2% BSA, 0.1% NaAz in PBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rat IgG-PE
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/200
Additional data
Additional Notes
Purple plot is secondary ab only (control), green is Wt mES cells, pink is Ecad-/- mES cells, published as having lower EPHa2 expression at the cell surface. I saw similar staining when using abcam's intracellular flow cytometry protocol (permeabilisation with tween20 prior to incubation with primary antibody).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 16 2013