Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (BEAS-2B (human bronchial epithelial cells))
Permeabilization
Yes - 0.1% Tween + 1% BSA + 10% FBS
Specification
BEAS-2B (human bronchial epithelial cells)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Methanol
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/10
Secondary antibody
Name
Non-Abcam antibody was used: universal, biotinylated anti-mouse/rabbit IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Use 10X dilution of this product could generate 100% positivity which was confirmed by using isotype control under the same condition. The signals were drastically reduced (mild positive) when using 200X dilution.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Yao Lee
Verified customer
提交于 Feb 12 2018