Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed
Additional data
Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immuno-fluorescently labeled and counterstained with DAPI. This antibody failed to produce the expected staining pattern under these conditions. MeOH-fixed samples were also evaluate and did not produce the expected staining pattern.
Abcam response
Thank you for submitting this Abreview. This antibody may not be suitable for this immunocytochemistry. It may require further optimization. I recommend a blocking step to reduce non-specific staining. Also, I recommend an antigen retrieval step as it may only recognize the denatured form of the protein, given that it's only been shown to work in WB.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 Apr 04 2014