Application
Flow Cytometry
Sample
Rat Cell (Brain)
Permeabilization
Yes - 0.25 % Triton X-100
Gating Strategy
Viable cells
Specification
Brain
Fixation
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+ 5% normal donkey serum
Dilution
1/100
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Allophycocyanin (APC) AffiniPure F(ab')2 Fragment
Host species: Donkey
Clonality: Polyclonal
Conjugation: Allophycocyanin
Host species: Donkey
Clonality: Polyclonal
Conjugation: Allophycocyanin
Additional data
Additional Notes
1 mL of 0.25 % Triton X-100 in PBS buffer was added to cells pellets in order to permeablize cells membrane. The samples were incubated for 30 minutes at room temperature with rocking, protected from light and washed once with 1 mL of PBS. Next, the cells were resuspended in blocking buffer containing 5% normal donkey serum in PBS and incubated for 2 hrs at room temperature with rocking, protected from light. The cell suspensions were stained overnight at 4 °C temperature with primary rabbit anti-ETB (1:100) protected from light with rocking.
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback about the use of ab117529 in rat samples and Flow Cytometry, a species and application that have not been tested yet. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Aneta Radziwon-Balicka
Verified customer
提交于 Oct 30 2017