Anti-Endothelin B Receptor/ET-B抗体(ab117529)
Key features and details
- Rabbit polyclonal to Endothelin B Receptor/ET-B
- Suitable for: IHC-Fr, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Endothelin B Receptor/ET-B抗体
参阅全部 Endothelin B Receptor/ET-B 一抗 -
描述
兔多克隆抗体to Endothelin B Receptor/ET-B -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, ICC/IF, IHC-Pmore details
不适用于: WB -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Goat, Horse, Chicken, Cow, Dog, Pig, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Mouse Endothelin B Receptor/ET-B aa 400-500 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P48302 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117529于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-Fr |
1/500 - 1/2000.
|
|
ICC/IF | (2) |
1/5000.
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IHC-P | (1) |
1/2000.
|
说明 |
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IHC-Fr
1/500 - 1/2000. |
ICC/IF
1/5000. |
IHC-P
1/2000. |
靶标
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功能
Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
组织特异性
Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells. -
疾病相关
Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily. -
翻译后修饰
Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 408082 Chicken
- Entrez Gene: 281750 Cow
- Entrez Gene: 100033875 Horse
- Entrez Gene: 1910 Human
- Entrez Gene: 13618 Mouse
- Entrez Gene: 100009477 Rabbit
- Omim: 131244 Human
- SwissProt: P28088 Cow
see all -
别名
- ABCDS antibody
- Ednra antibody
- EDNRB antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor/ET-B with ab117529.
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Immunohistochemistry (Frozen sections) analysis of mouse skin tissue sections labeling Endothelin B Receptor/ET-B with ab117529 at 1/800 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor/ET-B with ab117529 at 1/1500 dilution. Tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C. The tissue was incubated with Anti-Endothelin B Receptor antibody (ab117529) for 12 hours in PBS at 20°C. A biotin conjugated goat anti-rabbit secondary antibody at 1/200 dilution.
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ab117529, at 1/5000 dilution, staining Endothelin B Receptor/ET-B in ETB-transfected HEK293 cells by Immunocytochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (35)
ab117529 被引用在 35 文献中.
- Shi CJ et al. TGFβR-1/ALK5 inhibitor RepSox induces enteric glia-to-neuron transition and influences gastrointestinal mobility in adult mice. Acta Pharmacol Sin 44:92-104 (2023). PubMed: 35794374
- Bonnamour G et al. NR2F1 regulates a Schwann cell precursor-vs-melanocyte cell fate switch in a mouse model of Waardenburg syndrome type IV. Pigment Cell Melanoma Res 35:506-516 (2022). PubMed: 35816394
- Weigert A et al. Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system. Nat Commun 13:6078 (2022). PubMed: 36241617
- Zhang Y et al. Endothelin-1, over-expressed in SOD1G93A mice, aggravates injury of NSC34-hSOD1G93A cells through complicated molecular mechanism revealed by quantitative proteomics analysis. Front Cell Neurosci 16:1069617 (2022). PubMed: 36531135
- Del Rio D et al. Ovarian Cancer-Driven Mesothelial-to-Mesenchymal Transition is Triggered by the Endothelin-1/β-arr1 Axis. Front Cell Dev Biol 9:764375 (2021). PubMed: 34926453