Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Permeabilization
Yes - overnight in 70% ethanol at -20, and 30 min in 0.5% Triton-X
Specification
NIH3T3
Blocking step
Roche blocking reagent as blocking agent for 30 minute(s) · Concentration: 1.35% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 20°C · Diluent: Tris/saline + roche blocking reagent
Dilution
1/435
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Molecular Probes anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Fixative is 3.7% formaldehyde / 5% acetic acid
The overnight incubation in -20 is vital for getting a good signal
Cells shown are counterstained with DAPI
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Jonathan Houseley
Verified customer
提交于 Jul 02 2011