Anti-EXD2抗体(ab121236)
Key features and details
- Rabbit polyclonal to EXD2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-EXD2抗体
参阅全部 EXD2 一抗 -
描述
兔多克隆抗体to EXD2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment, corresponding to amino acids 335-442 of Human EXD2
-
阳性对照
- WB: A431, NIH/3T3 and NBT-II cell lysates. ICC/IF: A431 cells. IHC-P: Human cerebral cortex, kidney, prostate and testis tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121236于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 56 kDa.
|
|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. pH 6.0
|
说明 |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 56 kDa. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. pH 6.0 |
靶标
-
序列相似性
Belongs to the EXD2 family.
Contains 1 3'-5' exonuclease domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 55218 Human
- Entrez Gene: 97827 Mouse
- Entrez Gene: 362759 Rat
- SwissProt: Q9NVH0 Human
- SwissProt: Q8VEG4 Mouse
- Unigene: 533878 Human
-
别名
- 4930539P14Rik antibody
- C14orf114 antibody
- C85658 antibody
see all
图片
-
Anti-EXD2 antibody (ab121236) at 0.4 µg/ml + A431 cell lysate
Predicted band size: 56 kDa -
Immunohistochemical analysis of human prostate tissue labeling exd2 with ab121236. The data shows moderate to strong nuclear and cytoplasmic positivity in glandular cells.
-
Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab121236 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
Immunohistochemical analysis of human testis tissue labeling exd2 with ab121236. The data shows moderate to strong cytoplasmic positivity in seminiferous ducts.
-
Immunohistochemical analysis of human kidney tissue labeling exd2 with ab121236. The data shows moderate to strong cytoplasmic positivity in cells in glomeruli.
-
Immunohistochemical analysis of human cerebral cortex tissue labeling exd2 with ab121236. The data shows moderate to strong nuclear and cytoplasmic positivity in neurons.
-
All lanes : Anti-EXD2 antibody (ab121236) at 0.4 µg/ml
Lane 1 : NIH/3T3 cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 56 kDa
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab121236 尚未被引用在任何文献中。