概述

  • 产品名称
    Anti-ETS1抗体[EPR546(2)]
    参阅全部 ETS1 一抗
  • 描述
    兔单克隆抗体[EPR546(2)] to ETS1
  • 经测试应用
    适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human ETS1.

  • 阳性对照
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab109212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 50 kDa).
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能
      Transcription factor.
    • 序列相似性
      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
      Contains 1 PNT (pointed) domain.
    • 翻译后修饰
      Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
      Ubiquitinated; which induces proteasomal degradation.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
      • C ets 1 protein antibody
      • c-ets-1 antibody
      • ETS 1 antibody
      • Ets protein antibody
      • ETS proto-oncogene 1, transcription factor antibody
      • ETS1 antibody
      • ETS1 oncogene antibody
      • ETS1 protein antibody
      • ETS1_HUMAN antibody
      • EWSR 2 antibody
      • EWSR2 antibody
      • FLJ10768 antibody
      • Oncogene ETS1 antibody
      • P54 antibody
      • Protein C-ets-1 antibody
      • v ets avian erythroblastosis virus E2 oncogene homolog antibody
      • v ets avian erythroblastosis virus E2 oncogene homolog 1 antibody
      • v ets avian erythroblastosis virus E26 oncogene homolog 1 antibody
      • v ets erythroblastosis virus E26 oncogene homolog 1 antibody
      • v-ets erythroblastosis virus E26 oncogene homolog 1 antibody
      see all

    图片

    • Overlay histogram showing Jurkat cells stained with ab109212 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109212, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-ETS1 antibody [EPR546(2)] (ab109212) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Molt-4 cell lysate
      Lane 4 : PBMC lysate
      Lane 5 : HepG2 cell lysate
      Lane 6 : Jurkat cell lysate
      Lane 7 : Saos-2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 50 kDa

    文献

    This product has been referenced in:
    • Liu YN  et al. Loss of Androgen-Regulated MicroRNA 1 Activates SRC and Promotes Prostate Cancer Bone Metastasis. Mol Cell Biol 35:1940-51 (2015). WB ; Human . Read more (PubMed: 25802280) »
    • Luo W  et al. A balance between B cell receptor and inhibitory receptor signaling controls plasma cell differentiation by maintaining optimal Ets1 levels. J Immunol 193:909-20 (2014). Read more (PubMed: 24929000) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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