概述

  • 产品名称
  • 描述
    兔多克隆抗体to ERp57
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Monkey
  • 免疫原

    ERp57 from rat liver

  • 阳性对照
    • Rat liver microsomal preparation

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab13507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 57 kDa. This dilution detects Erp57 in 20µg of rat liver microsomal preparation by ECL analysis.
ICC/IF 1/1000.
IHC-P Use at an assay dependent concentration. PubMed: 21799787

靶标

图片

  • Anti-ERp57 antibody (ab13507) at 1/2000 dilution + Human LNCaP whole cell lysate at 25 µg

    Secondary
    HRP-conjugated Donkey anti-rabbit at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 45 seconds

    This image is courtesy of an Abreview submitted by Armen Petrosyan

    See Abreview

  • ab13507 staining ERp57 in the Human PC-3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab13507 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13507, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ERp57 antibody (ab13507) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from CHO-K1 cells
    Lane 3 : Cell lysates prepared from human liver microsomes
    Lane 4 : Cell lysates prepared from rat liver microsomes
    Lane 5 : Cell lysates prepared from mouse liver microsomes


    Predicted band size : 57 kDa

文献

This product has been referenced in:
  • Lea RG  et al. The fetal ovary exhibits temporal sensitivity to a 'real-life' mixture of environmental chemicals. Sci Rep 6:22279 (2016). IHC . Read more (PubMed: 26931299) »
  • Petrosyan A  et al. Keratin 1 plays a critical role in golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail. J Biol Chem 290:6256-69 (2015). Read more (PubMed: 25605727) »

See all 9 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Rat Cell lysate - other (Rat hepatocytes - Golgi fraction)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
20 µg
Specification
Rat hepatocytes - Golgi fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Nov 19 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Rat Cell (Rat hepatocytes)
Specification
Rat hepatocytes
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 29 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Human Cell (PC-3 - prostate cancer cells)
Specification
PC-3 - prostate cancer cells
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Nov 13 2013

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Sample
Human Cell lysate - whole cell (LNCaP)
Specification
LNCaP
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Nov 13 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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