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Our Abpromise guarantee covers the use of ab10287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 23762310
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration. PubMed: 20731657|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000 - 1/3000. Detects a band of approximately 57 kDa (predicted molecular weight: 61 kDa).|
|IP||Use at an assay dependent concentration.|
Western blot using ab10287 at 1/1000.
Lane 1 – Mouse Embryonic Fibroblasts.
Lane 2 – Calreticulin deficient Mouse Embryonic Fibroblasts.
ab10287 at 1/100 staining human HeLa cells by ICC/IF. The cells were premeabilized with digitonin before being blocked and then stained with the antibody for 1 hour. An Alexa-Fluor ® conjugated goat anti rabbit antibody was used as the secondary.
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