Application
Western blot
Sample
Human Cell lysate - whole cell (human pancreatic cancer cell lines)
Loading amount
30 µg
Specification
human pancreatic cancer cell lines
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
8 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Amersham-HRP Sheep anti-mouse
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: ca.60 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 05 2010