Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Baboon Tissue sections (Prepubertal testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
No
Specification
Prepubertal testis
Blocking step
5% serum + 3% BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Diluent: PBS 5% goat serum 3% BSA 0.1% Triton X
Dilution
1/100
Secondary antibody
Concentration
10 µg/ml
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
Immunoreactivity was evident prominently among germ cells in either nuclear or cytoplasmic localization, as has been reported previously. Some immunoreactivity (albeit lower intensity) also observed among Sertoli cells, as expected. Staining in adult testes was widespread among germ cells in nuclei (not shown).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 01 2017