重组Alexa Fluor® 488荧光Anti-ERK1 + ERK2抗体[EPR17526] (ab208564)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR17526] to ERK1 + ERK2
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 488荧光Anti-ERK1 + ERK2抗体[EPR17526]
参阅全部 ERK1 + ERK2 一抗 -
描述
Alexa Fluor® 488荧光兔单克隆抗体[EPR17526] to ERK1 + ERK2 -
宿主
Rabbit -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Also SwissProt ID P27361.
Database link: P28482 -
阳性对照
- ICC/IF: A431 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17526 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
- Alexa Fluor® 647 Anti-ERK1 + ERK2 antibody [EPR17526] (ab208881)
- HRP Anti-ERK1 + ERK2 antibody [EPR17526] (ab209321)
- Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)
- Alexa Fluor® 594 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210460)
- Alexa Fluor® 555 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210659)
- PE Anti-ERK1 + ERK2 antibody [EPR17526] (ab212153)
- Anti-ERK1 + ERK2 antibody [EPR17526] - BSA and Azide free (ab218017)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab208564于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100.
This product gave a positive signal in A431 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
说明 |
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ICC/IF
1/100. This product gave a positive signal in A431 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
靶标
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功能
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Omim: 176948 Human
- Omim: 601795 Human
see all -
形式
Mainly expressed in the cytoplasm and only localizes to the nucleus with treatment. -
别名
- ERK 1 antibody
- ERK 2 antibody
- ERK-1 antibody
see all
图片
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ab208564 staining ERK1 + ERK2 in A431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208564 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in A431 cells fixed with 4% formaldehyde (10 min).
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab208564 尚未被引用在任何文献中。