Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
None
Permeabilization
Yes - PBS-Triton X100 0.2%
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Overnight
Dilution
1/150
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Goat anti-Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Additional data
Additional Notes
Permeabilization 4% PFA. Blocking solution 5% BSA 5% Goat Serum.
Image of a postnatal day 1 mouse heart (low autofluorescence)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ricardo Santamaría
Verified customer
提交于 Apr 11 2017