Anti-EPS15R抗体[EP1147Y] (ab53006)
Key features and details
- Rabbit monoclonal [EP1147Y] to EPS15R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-EPS15R抗体[EP1147Y]
参阅全部 EPS15R 一抗 -
描述
兔单克隆抗体[EP1147Y] to EPS15R -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa cell lysate. IHC-P: Human breast carcinoma tissue.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP1147Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53006于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/50000. Detects a band of approximately 125 kDa (predicted molecular weight: 94 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/50000. Detects a band of approximately 125 kDa (predicted molecular weight: 94 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2. -
序列相似性
Contains 1 EF-hand domain.
Contains 3 EH domains. -
翻译后修饰
Phosphorylated on tyrosine residues by EGFR. -
细胞定位
Cell membrane. Nucleus. Membrane > coated pit. Localized to plasma membrane coated pits. - Information by UniProt
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数据库链接
- Entrez Gene: 58513 Human
- Omim: 600051 Human
- SwissProt: Q9UBC2 Human
- Unigene: 654639 Human
- Unigene: 744842 Human
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别名
- EP15R_HUMAN antibody
- epidermal growth factor receptor pathway substrate 15- ike 1 antibody
- Epidermal growth factor receptor substrate 15 like 1 antibody
see all
图片
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Anti-EPS15R antibody [EP1147Y] (ab53006) at 1/50000 dilution + HeLa cell lysate at 10 µg
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 94 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted? -
ab53006, at a 1/50 dilution, staining EPS15R in human breast carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab53006 被引用在 5 文献中.
- Day KJ et al. Liquid-like protein interactions catalyse assembly of endocytic vesicles. Nat Cell Biol 23:366-376 (2021). PubMed: 33820972
- Traub LM A nanobody-based molecular toolkit provides new mechanistic insight into clathrin-coat initiation. Elife 8:N/A (2019). PubMed: 31038455
- Evergren E et al. Eps15R and clathrin regulate EphB2-mediated cell repulsion. Traffic 19:44-57 (2018). PubMed: 28972287
- Liu Z & Zheng Y A requirement for epsin in mitotic membrane and spindle organization. J Cell Biol 186:473-80 (2009). PubMed: 19704019
- Chen C & Zhuang X Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus. Proc Natl Acad Sci U S A 105:11790-5 (2008). PubMed: 18689690