Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - other (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: ?
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 106 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
Untransfected Cell Lysate
Positive control
Transfected Cell Lysate
Additional data
Additional Notes
Lane
1 HEK293 cell lysate untransfected
2 HEK293 transfected with ENNP3 (99.8kD)
3 HEK293 transfected with ENPP1 (131kD)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Paul Stabach
Verified customer
提交于 Sep 12 2014