Anti-ENPP3/B10抗体[NP4D6] (ab90754)
Key features and details
- Mouse monoclonal [NP4D6] to ENPP3/B10
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-ENPP3/B10抗体[NP4D6]
参阅全部 ENPP3/B10 一抗 -
描述
小鼠单克隆抗体[NP4D6] to ENPP3/B10 -
宿主
Mouse -
特异性
The mouse monoclonal antibody NP4D6 reacts with CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human ENPP3/B10. HEK-293 cells transfected with human ENPP3/B10
Database link: O14638 -
阳性对照
- Flow Cyt: Human peripheral whole blood cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified from cell culture supernatant with > 95% purity (by SDS-PAGE) -
克隆
单克隆 -
克隆编号
NP4D6 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab90754于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
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说明 |
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Flow Cyt
Use a concentration of 1 - 4 µg/ml. |
靶标
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功能
Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD. -
组织特异性
Expressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors. -
序列相似性
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Contains 2 SMB (somatomedin-B) domains. -
翻译后修饰
N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. -
细胞定位
Membrane. Secreted. Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells. - Information by UniProt
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数据库链接
- Entrez Gene: 5167 Human
- Entrez Gene: 5169 Human
- Omim: 602182 Human
- SwissProt: O14638 Human
- SwissProt: P22413 Human
- Unigene: 486489 Human
- Unigene: 527295 Human
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别名
- Alkaline phosphodiesterase I antibody
- ARHR2 antibody
- B10 antibody
see all
图片
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Separation of CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of IgE stimulated human peripheral whole blood using ab90754 2 μg/ml.
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Flow cytometry surface staining pattern of IgE stimulated human peripheral whole blood stained using ab90754 at 2 μg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab90754 被引用在 2 文献中.
- Trapero C et al. Impaired Expression of Ectonucleotidases in Ectopic and Eutopic Endometrial Tissue Is in Favor of ATP Accumulation in the Tissue Microenvironment in Endometriosis. Int J Mol Sci 20:N/A (2019). PubMed: 31698766
- Aliagas E et al. Ecto-nucleotidases distribution in human cyclic and postmenopausic endometrium. Purinergic Signal 9:227-37 (2013). IHC-Fr ; Human . PubMed: 23225236