Anti-EME1抗体(ab88878)
Key features and details
- Mouse polyclonal to EME1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-EME1抗体 -
描述
小鼠多克隆抗体to EME1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human EME1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- HeLa or Jurkat cell lysate. HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88878于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. -
序列相似性
Belongs to the EME1/MMS4 family. -
细胞定位
Nucleus > nucleolus. Recruited to regions of DNA damage in S-phase cells. - Information by UniProt
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数据库链接
- Entrez Gene: 146956 Human
- Omim: 610885 Human
- SwissProt: Q96AY2 Human
- Unigene: 514330 Human
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别名
- 6820428D13 antibody
- Crossover junction endonuclease EME1 antibody
- EC 3.1.22.- antibody
see all
图片
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab88878 被引用在 2 文献中.
- Zhang H et al. TDP1-independent pathways in the process and repair of TOP1-induced DNA damage. Nat Commun 13:4240 (2022). PubMed: 35869071
- Guo Z et al. Essential meiotic structure-specific endonuclease1 (EME1) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway. Bioengineered 12:9869-9884 (2021). PubMed: 34719326