重组Anti-EIF2S1 (phospho S51)抗体[E90] (ab32157)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E90] to EIF2S1 (phospho S51)
- Suitable for: WB, IHC-P, Dot blot
- Reacts with: Mouse, Rat, Human, Neurospora crassa
Related conjugates and formulations
概述
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产品名称
Anti-EIF2S1 (phospho S51)抗体[E90]
参阅全部 EIF2S1 一抗 -
描述
兔单克隆抗体[E90] to EIF2S1 (phospho S51) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, Dot blotmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human, Neurospora crassa
预测可用于: Cow, Pig, Drosophila melanogaster, Monkey, Plants, African green monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human liver carcinoma, breast carcinoma, cervical carcinoma, colon adenocarcinoma and hepatocellular carcinoma tissues. WB: HeLa cells treated with Clyculin A and phosphatase whole cell lysate; PC-12 cell lysate. Dot Blot: Antigen peptide.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E90 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32157于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (19) |
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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IHC-P | (2) |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Dot blot |
1/500.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Dot blot
1/500. |
靶标
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功能
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. -
序列相似性
Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain. -
翻译后修饰
Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation. -
细胞定位
Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). - Information by UniProt
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数据库链接
- Entrez Gene: 327694 Cow
- Entrez Gene: 32617 Drosophila melanogaster
- Entrez Gene: 1965 Human
- Entrez Gene: 13665 Mouse
- Entrez Gene: 54318 Rat
- Omim: 603907 Human
- SwissProt: P41374 Drosophila melanogaster
- SwissProt: P05198 Human
see all -
别名
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
see all
图片
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All lanes : Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/1000 dilution
Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) Whole cell lysates
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates
Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates. Then the membrane was incubated with alkaline phosphatase.
Lane 4 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates. Then the membrane was incubated with lambda phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 180 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded human lung cancer sections labeling EIF2S1 with ab32157 at 1/4000 dilution (0.37 μg/mL). Hematoxylin was used as counterstain. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Antigen retrieval was heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on human lung cancer without alkaline phosphatase treatment (image A). No staining on human lung cancer with alkaline phosphatase treatment (image B)
The section was incubated with ab32157 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
Dot blot analysis of EIF2S1 (pS51) phospho peptide (Lane 1), EIF2S1 non-phospho peptide (Lane 2) with ab32157 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/20000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysates
Lane 2 : HeLa treated with Calyculin A whole cell lysates
Lane 3 : HeLa treated with Calyculin A and phosphatase whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDaBlocking buffer and concentration: 2% BSA/TBST.
Diluting buffer and concentration: 2% BSA /TBST.
Exposure time: 10 seconds
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Immunohistochemical analysis of paraffin-embedded mouse pancreatic cancer sections labeling EIF2S1 with ab32157 at 1/4000 dilution (0.37 μg/mL). Hematoxylin was used as counterstain. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Antigen retrieval was heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on mouse pancreatic cancer without alkaline phosphatase treatment (image A). No staining on mouse pancreatic cancer with alkaline phosphatase treatment (image B)
The section was incubated with ab32157 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab32157 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Dot blot analysis on antigen peptide. A nitrocellulose membrane was spotted with (1) phospho-peptide and (2) non-phospho-peptide at 5, 1, and 0.1 ng, and then blotted with ab32157 at 1:500 dilution.
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Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/500 dilution + PC12 cell lysate
Predicted band size: 36 kDa
Observed band size: 36 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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ab32157 showing positive staining in Breast carcinoma tissue at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab32157 showing positive staining in Cervical carcinoma tissue at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab32157 showing positive staining in Colonic adenocarcinoma tissue at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab32157 showing positive staining in Hepatocellular carcinoma tissue at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (241)
ab32157 被引用在 241 文献中.
- Amin PH et al. An RNA stem-loop functions in conjunction with an upstream open reading frame to direct preferential translation in the integrated stress response. J Biol Chem 299:102864 (2023). PubMed: 36596357
- Vasudevan D et al. A protein-trap allele reveals roles for Drosophila ATF4 in photoreceptor degeneration, oogenesis and wing development. Dis Model Mech 15:N/A (2022). PubMed: 34919148
- Wong DCS et al. CRYPTOCHROMES promote daily protein homeostasis. EMBO J 41:e108883 (2022). PubMed: 34842284
- Pietras P et al. Translation inhibition and suppression of stress granules formation by cisplatin. Biomed Pharmacother 145:112382 (2022). PubMed: 34864307
- Smirnova VV et al. Ribosomal leaky scanning through a translated uORF requires eIF4G2. Nucleic Acids Res 50:1111-1127 (2022). PubMed: 35018467