Anti-EIF2G/EIF2S3抗体(ab33207)
Key features and details
- Rabbit polyclonal to EIF2G/EIF2S3
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-EIF2G/EIF2S3抗体
参阅全部 EIF2G/EIF2S3 一抗 -
描述
兔多克隆抗体to EIF2G/EIF2S3 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Human -
免疫原
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阳性对照
- WB: NIH 3T3, PC12 and MEF1 whole cell lysates, mouse liver, spinal cord, testis, ovary, heart and kidney tissue lysates. ICC/IF: MEF1 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33207于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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说明 |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
靶标
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功能
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. -
序列相似性
Belongs to the GTP-binding elongation factor family. EIF2G subfamily. - Information by UniProt
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数据库链接
- Entrez Gene: 1968 Human
- Entrez Gene: 26905 Mouse
- Entrez Gene: 26908 Mouse
- Entrez Gene: 100312984 Rat
- Entrez Gene: 299027 Rat
- Omim: 300161 Human
- SwissProt: P41091 Human
- SwissProt: Q9Z0N1 Mouse
see all -
别名
- eIF-2-gamma X antibody
- eIF-2gA antibody
- eIF-2gX antibody
see all
图片
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ICC/IF image of ab33207 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33207, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-EIF2G/EIF2S3 antibody (ab33207) at 1 µg/ml
Lane 1 : Mouse Embryonic Fibroblast 1
Lane 2 :NIH/3T3 whole cell lysate (ab7179)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
ab33207 also gave a positive result in mouse adult brain, testes, heart, skeletal muscle, brain (0 day) and fetal (14 day) lysates. -
All lanes : Anti-EIF2G/EIF2S3 antibody (ab33207) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 4 : Heart (Mouse) Tissue Lysate
Lane 5 : Kidney (Mouse) Tissue Lysate
Lane 6 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 7 : Spinal Cord (Mouse) Tissue Lysate
Lane 8 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 9 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab33207 被引用在 1 文献中.
- de Almeida RA et al. A yeast purification system for human translation initiation factors eIF2 and eIF2Be and their use in the diagnosis of CACH/VWM disease. PLoS One 8:e53958 (2013). WB ; Human . PubMed: 23335982