重组Anti-EHD1抗体[EPR4955] (ab109747)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4955] to EHD1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-EHD1抗体[EPR4955]
参阅全部 EHD1 一抗 -
描述
兔单克隆抗体[EPR4955] to EHD1 -
宿主
Rabbit -
特异性
The immunogen used for this product shares 66% homology with EHD3. Cross-reactivity with this protein has not been confirmed experimentally.
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经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HepG2, HeLa and JAR cell lysates; HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4955 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109747于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 61 kDa.
|
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ICC/IF |
1/100 - 1/250.
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说明 |
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Flow Cyt (Intra)
1/1000 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 61 kDa. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. -
组织特异性
Highly expressed in testis. -
序列相似性
Contains 1 EF-hand domain.
Contains 1 EH domain. -
结构域
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins. -
细胞定位
Cell membrane. Early endosome membrane. Recycling endosome membrane. Associated with intracellular tubulovesicular structures. - Information by UniProt
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数据库链接
- Entrez Gene: 10938 Human
- Entrez Gene: 13660 Mouse
- Entrez Gene: 293692 Rat
- Omim: 605888 Human
- SwissProt: Q9H4M9 Human
- SwissProt: Q9WVK4 Mouse
- SwissProt: Q641Z6 Rat
- Unigene: 523774 Human
see all -
别名
- CDABP0131 antibody
- EH domain containing 1 antibody
- EH domain-containing protein 1 antibody
see all
图片
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Overlay histogram showing HeLa cells stained with ab109747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109747, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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All lanes : Anti-EHD1 antibody [EPR4955] (ab109747) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa -
ab109747, at 1/100 dilution, staining EHD1 in HeLa cells by Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab109747 被引用在 6 文献中.
- Sobu Y et al. Pathogenic LRRK2 regulates ciliation probability upstream of tau tubulin kinase 2 via Rab10 and RILPL1 proteins. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33653948
- Nakamura T et al. Functional and behavioral effects of de novo mutations in calcium-related genes in patients with bipolar disorder. Hum Mol Genet 30:1851-1862 (2021). PubMed: 34100076
- Vidal-Quadras M et al. Endocytic turnover of Rab8 controls cell polarization. J Cell Sci 130:1147-1157 (2017). PubMed: 28137756
- Matsudaira T et al. Endosomal phosphatidylserine is critical for the YAP signalling pathway in proliferating cells. Nat Commun 8:1246 (2017). PubMed: 29093443
- Kukic I et al. The IN/OUT assay: a new tool to study ciliogenesis. Cilia 5:23 (2016). PubMed: 27493724
- Lenhart KC et al. GRAF1 promotes ferlin-dependent myoblast fusion. Dev Biol 393:298-311 (2014). PubMed: 25019370