Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4-12%)
Loading amount
30 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1x TBS-T
Dilution
1/1000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 53 kDa Non-specific: 60 kDa
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提交于 Jun 03 2016